期刊论文

The toxic effects of free state N^ε-(Carboxymethyl)-lysine on C. elegans

中文

武汉轻工大学学报

2095-7386

2019

38

03

4-12+36

10.3969/j.issn.2095-7386.2019.03.002

食品污染物暴露组解析和总膳食研究(2017YFC1600503)； 武汉市居民膳食乳制品中羧甲基赖氨酸暴露水平的研究(2015y13)；

本校为第一机构

羧甲基赖氨酸(CML)是食品加工中经美拉德反应由蛋白质与糖类通过非酶催化反应产生的一种晚期糖基化终末产物,是一种新型食品潜在有害物质。本文通过研究外源游离性CML对秀丽隐杆线虫寿命、子代数量、热环境中线虫存活时间的影响,探讨其可能的毒性机制。结果表明,外源游离性CML导致线虫产卵期缩短,寿命试验以及热刺激下存活的线虫数量呈现出剂量-反应关系。影响了TOR信号通路的Skn-1基因和生殖系统信号通路中的hsf-1基因,提高线虫的热环境阻力的抵抗力并延长其寿命。高浓度的CML降低了线虫的中DAF-16的表达水平从而影响其寿命,抑制线虫体内Folt-1基因的表达,从而减少其子代线虫的数量。

N~ε-(Carboxymethyl)-lysine(CML) is an advanced glycation end product formed on protein by combined nonenzymatic glycation and oxidation(glycoxidation) reactions during the maillard reaction in food processing. In this study,the effects of exogenous free CML on the life span,the number of offspring and the thermal environment of C. elegans and the survival time of C. elegans at different concentrations were investigated,and the possible toxic mechanism was speculated. The results showed that CML resulted in shorter spawning period of nematode w...