期刊论文

中文

武汉轻工大学学报

2095-7386

2019

38

2

1-8

10.3969/j.issn.2095-7386.2019.02.001

国家重点研发计划(2017YFD0500600).

本校为第一机构

为研究一种W/O/W多重乳佐剂并包埋香菇多糖的制备工艺,试验采用自乳化方法结合低能乳化法,通过粒径和电位对混合乳化剂的HLB值,混合乳化剂含量,W/O初乳含量,剪切速度和剪切时间进行优化研究。结果表明:最适W/O/W多重乳佐剂制备条件为混合乳化剂HLB值为10,混合乳化剂含量为7%,W/O初乳含量为10%,剪切速度为10000 rpm,剪切时间为3 min,在此条件下所制乳液为乳白色均一液体,粒径为177. 1±3. 1 nm,Zeta电位为-27. 7±0. 3 mv,剂型鉴定为W/O/W型。所制W/O/W多重乳佐剂粘度小,包埋香菇多糖后理化性质较稳定,作为纳微米级W/O/W多重乳具有进一步作为疫苗佐剂的研究价值。

To study a preparation process of a W/O/W multiple emulsion adjuvant and embedding lentinan. The self-emulsification method combined with low-energy emulsification method was used to optimize the HLB value,by mixing emulsifier content, and W/O colostrum content, with shear rate and shear time of the mixed emulsifier by particle size and potential. The results showed that the optimal W/O/W multiple emulsion adjuvant preparation conditions were mixed emulsifier HLB value of 10, mixed emulsifier content of 7%, W/O colostrum content of 10%, shear r...