This study aimed at investigation of main microorganisms of smoked bacon during the shelf life, and providing reference data for the local bacon production. The smoked bacon produced in Shennongjia forestry district was used. The total counts of bacteria and fungi were detected and the freshness parameters (TVB-N and POV) were measured. The viable microorganisms were isolated by using PCA and MRS mediums and identified by 16SrDNA sequencing. The results showed that the test bacon maintained freshness as the total microbial counts and POV remained low levels. Three isolated strains were identif...