期刊论文

中文

武汉轻工大学学报

2095-7386

2019

38

2

9-15

10.3969/j.issn.2095-7386.2019.02.002

校企产学研项目(2015026).

本校为第一机构

选取平均体长为7. 8±1. 5 cm、平均体重为4. 45±0. 58 g的黄颡鱼幼鱼为试验对象。利用RT-PCR和RACE技术,测定NPY基因在黄颡鱼脑、肝、脾、肌肉4种组织中的相对表达量,并分析了在3种不同投喂策略下黄颡鱼幼鱼脑部NPY表达水平的变化,探讨百里香酚对黄颡鱼脑部NPY基因表达的影响。结果显示:黄颡鱼脑部的NPY基因表达量远远高于肝脏和脾脏,肌肉组织的NPY基因表达量最低。试验组Ⅰ在投喂基础饲料5 h后投喂百里香酚,NPY相对基因表达量出现明显上升（P

An experiment was conducted to evaluate the effect of different strategy of the thyme of phenol on tissue s NPY expression of yellow catfish juvenile. Yellow catfish were chosen with average length 7.8±1.5 cm and average weight 4.45±0.58 g to evaluate the effect of expression in brain tissue under 3 different feeding strategies. The NPY mRNA expression in 4 tissues (brain, liver, spleen, muscle) was determined by RT-PCR and RACE. The results showed that the NPY gene expression in brain was significantly higher than other tissues, and NPY expr...