期刊论文

中文

武汉轻工大学学报

2095-7386

2022

41

2

33-37

10.3969/j.issn.2095-7386.2022.02.006

本校为第一机构

研究昼夜节律异常对小鼠脾脏免疫功能的影响。采用14月龄的节律基因Per1/Per2双敲除(Per1-/-/Per2-/-)雄性C57BL/6小鼠（双敲除组，DKO）与野生型的雄性C57BL/6小鼠（野生组，WT）,测量体重、脾脏重量，同时，对脾脏组织进行HE染色、免疫组化、转录组测序以及脾脏B细胞和T细胞的流式细胞检测，分析节律基因敲除所导致的节律异常对小鼠脾脏免疫功能的影响。结果显示：与野生型相比，双敲除组脾脏指数增加，HE染色显示脾脏白髓变薄，动脉周围淋巴鞘消失且边缘区消失；流式细胞检测结果显示，双敲除组的B细胞和T细胞的比例都显著下降。TFR1免疫组化显示DKO小鼠出现铁死亡表现，转录组测序结果显示双...

To study the effect of abnormal circadian rhythm on spleen immune function of mice.Body weight,spleen weight and tissue morphology of 14-month-old male C57BL/6 mice with Per1/Per2 double knockout(DKO)and wild-type male C57BL/6 mice(wild group)were measured by HE staining,immunohistochemistry and transcriptome sequencing.At the same time,flow cytometry were performed on spleen B cells and T cells.To analyze the effect of abnormal rhythm induced by rhythm gene knockout on spleen immune function of mice.The results showed that compared with the wild type,spleen index was higher in the double knoc...