期刊论文

中文

武汉轻工大学学报

2095-7386

2022

41

4

35-42

10.3969/j.issn.2095-7386.2022.04.006

本校为第一机构

为研究红茶菌发酵黄芩(Scutellaria baicalensis Georgi)的抗菌作用和对果蝇及小鼠的细菌性肠道炎症的改善作用,测定黄芩发酵液的OD值、pH值等来确定红茶菌的发酵条件,采用二倍稀释法确定发酵液的最小抑菌浓度,使用金黄色葡萄球菌(Staphylococcus aureus)建立果蝇和小鼠的肠炎模型,通过果蝇存活率、小鼠空肠组织HE的染色情况、Zo-1和Occludin-1连接蛋白的免疫组化情况、小鼠空肠组织的荧光定量分析来验证发酵液的功能.结果显示,黄芩提取物在30℃下经红茶菌发酵84 h后,对2×108 CFU/mL的金黄色葡萄球菌的抑菌效果提升了8倍,喂食5％黄芩发酵液的果蝇生存率高于空白模型组及黄芩提取物组.小鼠肠道的...

In order to explore the effect of fermentation of Scutellaria baicalensis Georgi on antibacterial activity and on bacterial intestinal inflammation in drosophila and mice, the fermentation conditions of Kombucha tea were determined by measuring the OD value and pH value of Scutellaria baicalensis fermentation broth. The minimum inhibitory concentration of Kombucha fermentation broth was determined by double dilution method. Staphylococcus aureus was used to establish the enteritis model of drosophila and mice. The survival rate of drosophila, H...