期刊论文

A preliminary investigation into the mechanism of action of ITGB2 in neutrophil migration and macrophage development in zebrafish

中文

武汉轻工大学学报

2095-7386

2023

42

02

68-74

10.3969/j.issn.2095-7386.2023.02.010

武汉市2020年度应用基础前沿项目(2020020601012231).

本校为第一机构

通过构建ITGB2(整合素β2亚基)瞬时敲除斑马鱼模型,探讨ITGB2在中性粒细胞的迁移与巨噬细胞的发育中发挥的作用。使用CRISPR/Cas9技术设计敲除斑马鱼的ITGB2,然后利用苏丹黑染色观察瞬时敲除组和正常斑马鱼...展开更多 通过构建ITGB2(整合素β2亚基)瞬时敲除斑马鱼模型,探讨ITGB2在中性粒细胞的迁移与巨噬细胞的发育中发挥的作用。使用CRISPR/Cas9技术设计敲除斑马鱼的ITGB2,然后利用苏丹黑染色观察瞬时敲除组和正常斑马鱼的全身中性粒细胞的分布情况及切尾后中性粒细胞的迁移情况;进一步采用中性红染色观察瞬时敲除组和正常斑马鱼脑室巨噬细胞的早期发育情况及永久造血后脑室巨噬细胞的发育情况...

The role of ITGB2 in neutrophil migration and macrophage development was investigated by constructing an ITGB2 transient knockout zebrafish model.The CRISPR/Cas9 technique was used to construct an ITGB2 knockout in zebrafish,and then Sudan black staining was used to obs...MORE The role of ITGB2 in neutrophil migration and macrophage development was investigated by constructing an ITGB2 transient knockout zebrafish model.The CRISPR/Cas9 technique was used to construct an ITGB2 knockout in zebrafish,and then Sudan black staining was used to observe the whole body neutrophil distribution and neut...