期刊论文

The effect of overexpression of eptC on polymyxin resistance of Salmonella

中文

武汉轻工大学学报

2095-7386

2023

42

03

41-45+58

10.3969/j.issn.2095-7386.2023.03.006

江西省市场监督管理局科技计划项目(GSJK202104)。

本校为第一机构

沙门氏菌耐药性严重危害公共卫生。沙门氏菌A7不携带多粘菌素耐药基因mcr,但对多粘菌素B高耐药。将沙门氏菌A7 eptC基因全长克隆到pGEM-T Easy载体，测序验证无误后，经双酶切构建pkk223-eptC原核表达载体，分别转化E.coli DH5 α、沙门氏菌多粘菌素敏感株NC28和低耐药株NC141,并测定重组菌的多粘菌素耐药性以及生物膜。结果显示，重组菌对多粘菌素的耐药性均无变化，生物成膜能力均有提高，其中NC141重组菌生物成膜能力有显著提升。eptC基因过表达可以增加菌株的生物膜成膜能力，但并不能影响多粘菌素B耐药性。所做研究为沙门氏菌多粘菌素耐药性的机制研究提供理论依据。

The antibiotic resistance of Salmonella poses a serious threat to public health.Polymyxin is known as the"last line drug"for the treatment of infections caused by Gram negative bacteria.The mechanism of polymyxin resistance of Salmonella remains unknown.Salmon...MORE The antibiotic resistance of Salmonella poses a serious threat to public health.Polymyxin is known as the"last line drug"for the treatment of infections caused by Gram negative bacteria.The mechanism of polymyxin resistance of Salmonella remains unknown.Salmonella strain A7 does no...