期刊论文

中文

武汉轻工大学学报

2095-7386

2023

42

02

61-67

10.3969/j.issn.2095-7386.2023.02.009

国家自然基金面上项目(31770119).

本校为第一机构

目前，需要将能在高温、高盐和碱性等极端环境中稳定的淀粉酶利用于农业生产中。从云南腾冲热泉附近的土壤样品中分离得到一株菌株，经验证其为一株能够产淀粉酶的嗜热菌。通过测定该菌的16S rRNA基因序列，进行序列比对和构建系统发育树，结合菌落和菌体形态的观察，确定了该菌的分类学地位，并利用非发酵细菌生化鉴定研究了该菌的生理生化性质。结果表明，该菌株为革兰氏阳性细菌，属于地衣芽孢杆菌，命名为CHJ05;通过不同温度和不同pH条件下的生长实验，确定了该菌生长的最佳温度为45℃,最适pH为9,还能在10%NaCl的高盐环境中稳定生长。该菌所产的淀粉酶在30～40℃和pH6～10能保持较高活性，Z...

In industrial and agricultural production,amylase is needed with stable activity under extreme environment such as high temperature,high salt and alkaline.In this paper,a strain was isolated from soil samples near Tengchong hot spring in Yunnan Province and proved to be...MORE In industrial and agricultural production,amylase is needed with stable activity under extreme environment such as high temperature,high salt and alkaline.In this paper,a strain was isolated from soil samples near Tengchong hot spring in Yunnan Province and proved to be a...