期刊论文

Extraction of protein from Pharbitidis semen and evaluation of anti-tumor activity

中文

武汉轻工大学学报

2095-7386

2023

42

02

75-81

10.3969/j.issn.2095-7386.2023.02.011

湖北省科技厅自然科学基金面上项目(2019CFB686).

本校为第一机构

利用响应面法对牵牛子黑丑的总蛋白提取进行工艺优化并观察其抗肿瘤活性。采用单因素及响应面法,以牵牛子蛋白提取量为指标,考察料液比、温度和pH三个因素对蛋白质提取量的影响。在经过双酶解法、SephadexG-15凝胶层析后,...展开更多 利用响应面法对牵牛子黑丑的总蛋白提取进行工艺优化并观察其抗肿瘤活性。采用单因素及响应面法,以牵牛子蛋白提取量为指标,考察料液比、温度和pH三个因素对蛋白质提取量的影响。在经过双酶解法、SephadexG-15凝胶层析后,利用MTT(噻唑蓝)实验观察多肽组分的抗肿瘤活性。响应面优化结果发现影响牵牛子总蛋白质提取的各因素主次顺序为料液比>温度>pH,且最佳提取工艺...

Objective:To optimize the extraction process of total protein from Pharbitidis semen and observe its anti-tumor activity by response surface methodology.Methods:Taking the extracted amount of Pharbitidis semen protein as the index,single factor and response surface meth...MORE Objective:To optimize the extraction process of total protein from Pharbitidis semen and observe its anti-tumor activity by response surface methodology.Methods:Taking the extracted amount of Pharbitidis semen protein as the index,single factor and response surface method...