期刊论文

中文

武汉轻工大学学报

2095-7386

2024

43

01

17-27

10.3969/j.issn.2095-7386.2024.01.003

本校为第一机构

2,4-二甲基苯酚作为许多工农业产品生产的中间体在环境中大量积累,由于其难以自然降解,造成了一定的环境污染,是一种顽固性的环境污染物.本研究从市政污水处理厂的活性污泥中分离出了一株能够高效降解2,4-二甲基苯酚的菌株4302,它能在24 h内降解2 mmol/L的2,4-二甲基苯酚,并基于单因素实验结果进行响应面实验优化菌株4302生长条件,结果表明:预测菌株4302生长的最适条件为:温度31.7 ℃、pH 7.4、底物浓度0.9 mmol/L,接种后培养14 h,在此条件下,OD600在理论上可达到0.117.酶活测定表明,菌株4302具有原儿茶酸3,4-双加氧酶酶活,未检测到邻苯二酚 2,3-双加氧酶和龙胆酸1,2-双加氧酶的酶活,说明2,4-二...

As an intermediate in producing many industrial and agricultural products,2,4-dimethylphenol accumulates in large quantities in the environment.Due to its difficulty in natural degradation,it causes par-ticular environmental pollution and is an essential ecological priority pollutant.In this study,a strain 4302,which can efficiently degrade 2,4-dimethylphenol,was isolated from the activated sludge of a wastewater treatment factory.After morphological observation,biochemical identification,and 16S rRNA sequence a-nalysis,the strain was identifie...