期刊论文

中文

武汉轻工大学学报

2095-7386

2024

43

2

50-55

10.3969/j.issn.2095-7386.2024.02.007

国家重点研发国际合作项目(编号:2023YFE0199200) 武汉市科技局2020年度应用基础前沿项目(编号:2020020601012231).

本校为第一机构

探究斑马鱼低氧诱导因子-1α(Hif-1α)基因在中性粒细胞和巨噬细胞的发育和迁移中所起的作用。将斑马鱼分为野生对照组和Hif-1α基因突变组。使用CRISPR/Cas9技术对斑马鱼的Hif-1α基因进行突变。随后,通过...展开更多 探究斑马鱼低氧诱导因子-1α(Hif-1α)基因在中性粒细胞和巨噬细胞的发育和迁移中所起的作用。将斑马鱼分为野生对照组和Hif-1α基因突变组。使用CRISPR/Cas9技术对斑马鱼的Hif-1α基因进行突变。随后,通过苏丹黑B染色法观察了野生对照组和Hif-1α基因突变组中性粒细胞的染色情况,以及通过中性红染色法观察了巨噬细胞的染色情况并对结果进行了统计分析。结果显示成功构建缺失8个...

Investigating the role of the zebrafish hypoxia-inducible factor-1 alpha(Hif-1α)gene in the development and migration of neutrophils and macrophages.Zebrafish were divided into wild-type control group and Hif-1αgene mutant group.CRISPR/Cas9 technology was utilized to mu...MORE Investigating the role of the zebrafish hypoxia-inducible factor-1 alpha(Hif-1α)gene in the development and migration of neutrophils and macrophages.Zebrafish were divided into wild-type control group and Hif-1αgene mutant group.CRISPR/Cas9 technology was utilized to ...