期刊论文

Construction and optimization of synthetic pathway of naringin from Saccharomyces cerevisiae

中文

武汉轻工大学学报

2095-7386

2024

43

4

49-57

10.3969/j.issn.2095-7386.2024.04.006

本校为第一机构

本研究构建酿酒酵母柚皮素细胞合成工厂,优化关键合成步骤,以提升柚皮素产量。采用同源重组技术,在酿酒酵母的rDNA位点构建从对香豆酸到柚皮素的合成途径,包括4-香豆酸辅酶A连接酶(4CL)、查尔酮合成酶(CHS)、查尔酮异...展开更多 本研究构建酿酒酵母柚皮素细胞合成工厂,优化关键合成步骤,以提升柚皮素产量。采用同源重组技术,在酿酒酵母的rDNA位点构建从对香豆酸到柚皮素的合成途径,包括4-香豆酸辅酶A连接酶(4CL)、查尔酮合成酶(CHS)、查尔酮异构酶(CHI)三个酶基因的完整表达阅读框。同时,单因素法优化YPD发酵培养基中酵母提取物,葡萄糖和胰蛋白胨浓度。结果表明:得到了5株产柚皮素的酿酒酵母改...

In this study,a naringenin cell synthesis plant of Saccharomyces cerevisiae was constructed to optimize the key synthetic steps and increase naringenin production.A synthetic pathway from para-coumaric acid to naringin was constructed at the rDNA site of Saccharomyces c...MORE In this study,a naringenin cell synthesis plant of Saccharomyces cerevisiae was constructed to optimize the key synthetic steps and increase naringenin production.A synthetic pathway from para-coumaric acid to naringin was constructed at the rDNA site of Saccharomyces cerevisiae using homologous recombination technology,...