期刊论文

中文

武汉轻工大学学报

2095-7386

2024

43

03

59-65

10.3969/j.issn.2095-7386.2024.03.009

国家自然科学基金青年基金项目(编号：31901081)； 中国博士后科学基金项目(编号：2019M662746)；

本校为第一机构

浒苔绿潮爆发带来了一系列环境问题,也对浒苔资源的开发利用提出了新挑战.从生物酶法降解浒苔的主要成分浒苔多糖入手,筛选出能降解浒苔多糖的菌株,对其进行16S rRNA基因鉴定,同时对其中表达量上调的木聚糖酶基因(GH43)进行异源表达,利用紫外-可见分光光度计比色法进行酶活测定及酶学性质研究.结果表明筛选到的浒苔多糖降解菌为黄杆菌(Algibacter pacificus),克隆表达的木聚糖酶的最适温度为50 ℃,最适pH约为5,粗酶液酶活为65.71 U/mL.实验可为由浒苔引起的海洋污染治理问题提供理论依据,同时为该菌株及木聚糖酶的工业化利用提供了参考,有利于实现对浒苔的资源化利用.

The outbreak of the green tide of Enteromorpha prolifera has brought a series of environmental problems and also posed new challenges to the development and utilization of Enteromorpha prolifera re-sources.Polysaccharides from Enteromorpha prolif era are the main components of Enteromorpha prolif-era.Starting with the enzymatic degradation of Enteromorpha prolifera polysaccharides,strains capable of degrading Enteromorpha prolif era polysaccharides were screened,and their 16S rRNA genes were identi-fied.At the same time,the upregulated xylanase...