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Production and Functional Characterization of a Novel Mannanase from Alteromonadaceae Bacterium Bs31

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成果类型:
期刊论文
作者:
Ding, Rui;Xie, Huifang;Han, Zhenggang;Yang, Jiangke
作者机构:
[Ding, Rui; Xie, Huifang; Yang, Jiangke; Han, Zhenggang] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
语种:
英文
关键词:
Auto-induction;Escherichia coli expression;glycoside hydrolase family 5;mannanase;mannooligosaccharides;Pichia pastoris expression.
期刊:
PROTEIN AND PEPTIDE LETTERS
ISSN:
0929-8665
年:
2022
卷:
29
期:
8
页码:
692-701
基金类别:
China Ocean Mineral Resources Research and Development Association [DY135-B2-06]
机构署名:
本校为第一机构
院系归属:
生命科学与技术学院
摘要:
Background: Mannans are the main components of hemicellulose in nature and serve as the major storage polysaccharide in legume seeds. To mine new mannanase genes and identify their functional characteristics are an important basis for mannan biotechnological applications.Objective: In this study, a putative mannanase gene (ManBs31) from the genome of the marine bacterium Alteromonadaceae Bs31 was characterized.Methods: Amino acid sequence analysis and protein structural modeling were used to reveal the molecular features of ManBs31. The catalyt...

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