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Enhanced production of recombinant calf chymosin in Kluyveromyces lactis via CRISPR-Cas9 engineering

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成果类型:
期刊论文
作者:
Zheng, Yanli;Wang, Shiqing;Deng, Yuhui;Hu, Ping;Xue, Qingxin;...
通讯作者:
Yang, JK;Chan, ZH;Peng, WF
作者机构:
[Yang, Jiangke; Wang, Shiqing; Zheng, Yanli; Deng, Yuhui; Hu, Ping; Lei, Lei] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan 430023, Peoples R China.
[Chan, Zhuhua; Hu, Ping] Marine Biol Resources Dev & Utilizat Engn Technol, TIO, MNR, Xiamen 361005, Fujian, Peoples R China.
[Peng, Wenfang; Peng, WF; Xue, Qingxin; Li, Jiaxin] Hubei Univ, Hubei Engn Res Ctr Bioenzyme Catalysis, Environm Microbial Technol Ctr Hubei Prov, Sch Life Sci,State Key Lab Biocatalysis & Enzyme E, Wuhan 430062, Peoples R China.
通讯机构:
[Yang, JK ] W
[Peng, WF ] H
[Chan, ZH ] M
Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan 430023, Peoples R China.
Marine Biol Resources Dev & Utilizat Engn Technol, TIO, MNR, Xiamen 361005, Fujian, Peoples R China.
语种:
英文
关键词:
CRISPR-Cas9;High-yield and industrial-scale;Kluyveromyces lactis;Precision strain engineering;Recombinant calf chymosin;Secretory production
期刊:
Bioresource Technology
ISSN:
0960-8524
年:
2025
卷:
419
页码:
132116
基金类别:
Acknowledgements This work was finally supported by the Key R&D Projects in Hubei Province, China (2021BCA113), the Open Project of the Technology Innovation Center for Exploitation of Marine Biological Resources, MNR ( TICMBR202201 ), and the Innovation Base for Introducing Talents of Discipline of Hubei Province ( 2019BJH021 ). WP and YZ also acknowledge the support from the State Key Laboratory of Biocatalysis and Enzyme Engineering . Authors’ contributions WP, YZ, JY, and ZC designed the research; YZ, SW, YD, PH, QX, JL and LL
机构署名:
本校为第一且通讯机构
院系归属:
生命科学与技术学院
摘要:
As an important industrial enzyme, chymosin has been widely used in cheese manufacturing. Fermentation with Kluyveromyces lactis has allowed recombinant chymosin production to fit the growing global demand for cheese consumption; yet improvements can be made to allow for stable and larger-scale production. In this work, various c hymosin p roducing (CP) strains were constructed via targeted chromosomal integration of various copies of a p rochymosin e xpression c assette (PEC) using a CRISPR-Cas9 platform optimized for K. lactis. It enabled the demonstration that chymosin yields could be incre...

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