Biochemical characterization of a new β-agarase from cellulophaga algicola

首页 > 成果 > 详情

认领

导出

Link by DOI

反馈

作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Han, Zhenggang;Zhang, Yuxi;Yang, Jiangke*

通讯作者：

Yang, Jiangke

作者机构：

[Yang, Jiangke; Han, Zhenggang; Zhang, Yuxi] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构：

[Yang, Jiangke] W

Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

语种：

英文

关键词：

agar;agarose;beta agarase;dithiothreitol;neoagarohexaose;neoagarotetraose;peptides and proteins;unclassified drug;agarase;bacterial protein;glycosidase;protein binding;affinity chromatography;alga;amino acid sequence;Article;bacterium;Cellulophaga Algicola;controlled study;enzyme activity;enzyme stability;Gracilaria lemaneiformis;hydrolysis;maximum reaction velocity;Michaelis constant;molecular cloning;molecular weight;nonhuman;pH;Porphyra haitanensis;protein analysis;protein expression;protein purification;salt tolerance;sequence alignment;sequence analysis;sequence homology;temperature;thermostability;thin layer chromatography;Zobellia galactanivorans;chemistry;enzyme active site;enzyme specificity;enzymology;Flavobacteriaceae;metabolism;Bacterial Proteins;Catalytic Domain;Enzyme Stability;Flavobacteriaceae;Glycoside Hydrolases;Protein Binding;Substrate Specificity

期刊：

International Journal of Molecular Sciences

ISSN：

1661-6596

年：

2019

卷：

期：

页码：

2143

DOI：

10.3390/ijms20092143

基金类别：

China Ocean Mineral Resources Research and Development Association#&#&#DY135-B2-06)

机构署名：

本校为第一且通讯机构

院系归属：

生命科学与技术学院

摘要：

Cellulophaga algicola DSM 14237, isolated from the Eastern Antarctic coastal zone, was found to be able to hydrolyze several types of polysaccharide materials. In this study, a predicted β-agarase (CaAga1) from C. algicola was heterologously expressed in Escherichia coli. The purified recombinant CaAga1 showed specific activities of 29.39, 20.20, 14.12, and 8.99 U/mg toward agarose, pure agar, and crude agars from Gracilaria lemaneiformis and Porphyra haitanensis, respectively. CaAga1 exhibited an optimal temperature and pH of 40 °C and 7, re...

反馈

产权有误：本人成果被他人认领

数据有误：数据基本信息有误

归属有误：成果的院系归属、机构署名归属有误

其他原因：

验证码：

看不清楚，换一个

确定

取消

成果认领

标题：

用户	作者	通讯作者	--
	请选择	请选择	--

确定

取消

Biochemical characterization of a new β-agarase from cellulophaga algicola

反馈

成果认领

提示

该栏目需要登录且有访问权限才可以访问