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Accurate and online quantification of viable Rhodobacter sphaeroides cells using a flow cytometry-dielectric spectroscopy (FCM-DS) method

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成果类型:
期刊论文
作者:
Wang, Meng;Liu, Siyuan;Wang, Zejian;Li, Bo;Li, Longquan;...
通讯作者:
Zejian Wang<&wdkj&>Zhiwei Sui
作者机构:
[Liu, Siyuan; Li, Longquan; Sui, Zhiwei; Wang, Meng; Wang, Ziquan] Natl Inst Metrol, Ctr Adv Measurement Sci, 18 Beisanhuandonglu, Beijing 100029, Peoples R China.
[Wang, Zejian; Li, Bo] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China.
[Li, Longquan; Zhou, Guoping] Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan, Peoples R China.
通讯机构:
[Zejian Wang] S
[Zhiwei Sui] C
Center for Advanced Measurement Science, National Institute of Metrology, Beijing, China<&wdkj&>State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
语种:
英文
关键词:
Coenzyme Q10;Dielectric spectroscopy;FCM-DS method;Flow cytometry;Rhodobacter sphaeroides;Viable cell count
期刊:
Talanta
ISSN:
0039-9140
年:
2022
卷:
245
页码:
123448
基金类别:
CRediT author statement Zhiwei Sui: acquisition, Methodology, Supervision. Zejian Wang: Conceptualization, Methodology, Resources. Meng Wang: Methodology, Investigation, Writing, Visualization. Siyuan Liu: Methodology, Investigation. Longquan Li: Methodology, Investigation. Bo Li: Methodology, Investigation. Ziquan Wang: Investigation. Guoping Zhou: Resources.
机构署名:
本校为其他机构
摘要:
Accurate and online quantification of viable cells is one of the necessary requirements during the microbial fermentation process for high productivity. The flow cytometry (FCM)-based method accurately quantifies viable cells, but this offline method cannot reflect the counts constantly. The dielectric spectroscopy (DS) sensor is widely utilized to monitor viable cells online; however, accurately converting the capacitance value of the DS sensor to the viable bacterial cell counts has barely been tried. We have developed a method by coupling th...

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