Polystyrene microspheres were prepared by the emulsifier-free polymerization method. Using the polystyrene microspheres as seeds, p-nitroaniline as template molecules, the monodispersed molecularly imprinted polymer microspheres (MIPMs) were prepared by a single-step swelling and polymerization method. The monodispersed molecular imprinted polymers with porous structure were characterized by SEM, and UV-visible spectrophotometry. The adsorpti...