期刊论文

EFFECTS OF DIETARY CLA ON CYTOLOGICAL MORPHOLOGY, ACTIVITY OF ANTIOXIDANT ENZYMES, AND GENE EXPRESSIONS OF LIPID METABOLISM IN THE LIVER AND MUSCLE OF GRASS CARP

Dong, G.-F.

中文

水生生物学报

1000-3207

2019

43

4

708-722

10.7541/2019.084

基金项目: 国家自然科学基金(31672666和31302195); 湖北省自然科学基金面上项目(2016CFB389); 湖北省教育厅重点项目 (D20151705); 动物营养与饲料安全湖北省协同创新中心资助 [Supported by the National Natural Science Foundation of China (31672666, 31302195); the Natural Science Foundation of Hubei Province of China (2016CFB389); Educational Commission of Hubei Province (D20151705); Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety] 作者简介: 孔龙(1994—), 男, 湖北孝感人; 硕士研究生; 主要从事水生动物营养与饲料研究。E-mail: kolo5247@outlook.com; 邹琦 (1991—), 男, 湖北洪湖人; 硕士研究生; 主要从事水生动物营养与饲料研究。E-mail: QiZou2019@126.com *共同第一作者 通信作者: 董桂芳(1980—), 女, 湖北孝感人; 博士; 主要从事水生动物营养与饲料研究。E-mail: dongguifang2002@126.com

本校为第一且通讯机构

采用65d的生长实验研究共轭亚油酸（CLA）对草鱼肝脏和肌肉细胞学形态、抗氧化能力以及脂肪代谢相关基因表达的影响。共配制7种近似等氮（粗蛋白:36 g/100 g）等脂（粗脂肪:4.5 g/100 g）的饲料:0 （对照组）、0.5 （CLA0.5）、1 （CLA1）、1.5 （CLA1.5）、2 （CLA2）、2.5 （CLA2.5）和3 g/100 g CLA （CLA3）。每个饲料组均设置3个生物学重复,实验鱼初始体重约为（5.08±0.08） g。实验结果显示:与对照组相比, CLA0.5-CLA2.5组草鱼肝脏和肌肉组织细胞学形态均无明显异常,但CLA3组肝细胞线粒体空泡化,内质网肿胀,排列过于紧密;肌细胞肌节结构松散,肌原纤维降解;在CLA1.5-CLA2.5组肝脏和肌肉中...

A 65-day trial was conducted to investigate effects of CLA on cytological morphology, activity of antioxidant enzymes, and gene expressions of lipid metabolism in the liver and muscle of grass carp. Seven isonitrogenous (crude protein: 36 g/100 g) and isolipidic (crude lipid: 4.5 g/100 g) diets were formulated: 0 CLA (control), 0.5% (CLA0.5), 1% (CLA1), 1.5 (CLA1.5), 2 (CLA2), 2.5 (CLA2.5), and 3% CLA (CLA3). Three biological replicates of fish were assigned to each diet with the initial body weight at (5.08±0.08) g. Endoplasmic reticulum in t...