期刊论文

Study on Methods of Measuring and Inducing Apoptosis Rapidly

中文

生物技术通讯

1009-0002

2013

1

83-86

10.3969/j.issn.1009-0002.2013.01.019

本校为第一机构

目的:探讨Ca2+和Na+诱导细胞凋亡的最佳浓度及时间,并用甲基绿-派诺宁染色法检测凋亡细胞的形态变化.方法:分别用不同浓度梯度及时间梯度的Ca2+和Na+胁迫处理洋葱鳞茎内表皮细胞,得诱导的最佳浓度及时间;用甲基绿-派诺宁染色法检测诱导凋亡的洋葱鳞茎内表皮细胞、大蒜根尖细胞和鸡血红细胞的形态特征变化.结果:诱导处理的最佳Ca2+和Na+浓度为0.4 mol/L,最适时间约为8 h,且CaCl2的诱导效果较NaCl好;经甲基绿-派诺宁染色,洋葱鳞茎内表皮细胞、大蒜根尖细胞、鸡血红细胞凋亡细胞的细胞核均呈蓝紫色,细胞质呈红色.结论:找出了诱导细胞凋亡的最适Ca2+和Na+浓度和时间,并检测到细胞凋亡.

Objective： To study the optimum concentration and time under Ca2＋ and Na＋, and detect the chang- es of apoptosis morphological characteristics by methyl green-pyronin staining. Methods： To get the optimum con- centration and the optimum time, onion skin cells within the bulb were observed and counted under the micro- scope after different concentration and different time of ions stress treatment. The methyl green-pyronin staining was used to detect the changes of induced apoptosis morphological characteristics for onion skin cells within th...