Development of a counterselectable system for rapid and efficient CRISPR-based genome engineering in Zymomonas mobilis

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成果类型：

期刊论文

作者：

Zheng, Yanli;Fu, Hongmei;Chen, Jue;Li, Jie;Bian, Yuejie;...

通讯作者：

Yang, JK;Liu, YH;Peng, WF

作者机构：

[Yang, Jiangke; Fu, Hongmei; Zheng, Yanli; Chen, Jue; Hu, Ping; Lei, Lei] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan 430023, Peoples R China.

[Liu, Yihan; Chen, Jue; Li, Jie] Tianjin Univ Sci & Technol, Coll Biotechnol, Key Lab Ind Fermentat Microbiol, Tianjin Key Lab Ind Microbiol,Minist Educ, Tianjin 300457, Peoples R China.

[Peng, Wenfang; Peng, WF; Bian, Yuejie; Li, Jie] Hubei Univ, Hubei Engn Res Ctr Bioenzyme Catalysis, Environm Microbial Technol Ctr Hubei Prov, Sch Life Sci,State Key Lab Biocatalysis & Enzyme E, Wuhan 430062, Peoples R China.

通讯机构：

[Yang, JK ] W

[Peng, WF ] H

[Liu, YH ] T

Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan 430023, Peoples R China.

Tianjin Univ Sci & Technol, Coll Biotechnol, Key Lab Ind Fermentat Microbiol, Tianjin Key Lab Ind Microbiol,Minist Educ, Tianjin 300457, Peoples R China.

语种：

英文

关键词：

Zymomonas mobilis;CRISPR-Cas genome engineering;Scarless mutagenesis;clmPheS counterselection marker;Multi-round multiplex genome editing;Native plasmids editing

期刊：

Microbial Cell Factories

ISSN：

1475-2859

年：

2023

卷：

期：

页码：

1-10

DOI：

10.1186/s12934-023-02217-9

基金类别：

This work was supported by The National Key Research and Development Program of China (2021YFC2100300), The Key R&D projects in Hubei Province, China (2021BCA113), The Innovation Base for Introducing Talents of Discipline of Hubei Province (2019BJH021), and Open Project Funding of the State Key Laboratory of Biocatalysis and Enzyme Engineering (SKLBEE2020021).

机构署名：

本校为第一且通讯机构

院系归属：

生命科学与技术学院

摘要：

Zymomonas mobilis is an important industrial bacterium ideal for biorefinery and synthetic biology studies. High-throughput CRISPR-based genome editing technologies have been developed to enable targeted engineering of genes and hence metabolic pathways in the model ZM4 strain, expediting the exploitation of this biofuel-producing strain as a cell factory for sustainable chemicals, proteins and biofuels production. As these technologies mainly take plasmid-based strategies, their applications would be impeded due to the fact that curing of the extremely stable plasmids is laborious and ineffic...

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Development of a counterselectable system for rapid and efficient CRISPR-based genome engineering in Zymomonas mobilis

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