National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31400556, 31370680]; Natural Science Foundation of Hubei ProvinceNatural Science Foundation of Hubei Province [2013CFB471]; Open Research Foundation of Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratories [2013000203]
机构署名:
本校为第一且通讯机构
院系归属:
生命科学与技术学院
摘要:
Ginkgolides are key pharmaceutical components in Ginkgo biloba. Using the cDNA sequence of the MECP and MECT genes to design primers, we obtained the promoters of these genes from Ginkgo genomic DNA using the genome walking method. The two promoters were 744 and 982 bp in length, respectively. The cis-elements of the GbMECPs and GbMECT promoters were predicted and analyzed using the plant cis-acting regulatory element database. We found major cis-elements in the sequence of the GbMECT and GbMECPs promoters. The GbMECP promoter contains six TATA...