期刊论文

中文

食品工业科技

1002-0306

2012

33

15

181-186

国家高技术研究发展计划(863计划)项目(2010AA023003)；

本校为第一机构

用分步酶解法制备鲢鱼抗氧化肽,首先从碱性蛋白酶、中性蛋白酶、胰蛋白酶、风味蛋白酶中筛选出碱性蛋白酶作为第一步酶解用酶,以水解度作评价指标,并得到酶解工艺条件:底物浓度15%,pH9.0,温度50℃,加酶量为24AU/kg。再从剩下的三种酶中筛选出胰蛋白酶与碱性蛋白酶复配,作为第二步酶解用酶,用响应面法进一步优化酶解工艺,考虑到成本和工艺的要求,最终确定的酶解工艺为:碱性蛋白酶水解时间为59.12min,胰蛋白酶水解时间为82.24min,两种酶的添加比例为1:1.48,即碱性蛋白酶添加量为24AU/kg,胰蛋白酶的添加量为35.5AU/kg。最终得到的鲢鱼抗氧化肽可溶性氮70.84%,多肽69.38%,水分4.21%,灰分25.67%;多肽...

In order to obtain Hypophthalmichthys molitrix antioxidant peptides,two-step-hydrolysis methodology was used.First of all,Alcalase was chosen as the enzyme for the first-step-hydrolysis from Alcalase,Flavourzyme,Trypsin and Neutrase.Degree of hydrolysis（DH）was tested by pH-stat method.Results indicated that the optimal processing conditions for hydrolyzing Hypophthalmichthys molitrix protein hydrolysate of Alcalase were protein concentrations 15%,pH9.0,50℃,ratio of Alcalase to silver carp protein with 24AU/kg.Then Trypsin was chosen from Fla...