期刊论文

Structural Characterization and Promoting Cell Proliferation of Collagen Oligopeptide Chelated Calcium from Silver Carp Fish Bone

Liu, Liangzhong(liu2022888@163.com)

中文

中国食品学报

1009-7848

2016

16

9

185-191

10.16429/j.1009-7848.2016.09.025

国家自然科学基金项目（21076166） 湖北省教育厅高校产学研合作资助项目（C2010040）

本校为第一且通讯机构

为开发一种具有良好吸收性和高利用率的补钙产品———胶原低聚肽螯合钙, 探究其结构特征及其对细胞的促生长作用。分别采用傅里叶红外光谱、紫外光谱、荧光光谱和差示热量扫描仪对胶原低聚肽螯合钙冻干样品进行表征分析,利用293Ad5+人肾细胞进行增殖培养试验,结果表明:胶原低聚肽中氨基上的氮原子与羧基上的氧原子均参与了钙离子的螯合反应,它们之间主要是配位结合。差示热量扫描(DSC)曲线表明:胶原低聚肽螯合钙具有比胶原低聚肽更好的稳定性。采用MTT法检测不同剂量组胶原低聚肽螯合钙对293Ad5+人肾细胞培养24h的相对增殖活力,结果表明胶原低聚肽螯合钙安全性高,在一定程度上促进了细胞的增殖...

In recent years, the problem of insufficient calcium nutrition of residents received has been widespread concerned in our country. In order to develop an easy digested and high utilized calcium product-collagen oligopeptide chelated calcium. And structure characteristics of collagen oligopeptide chelated calcium are explored and they effect on promoting cell proliferation. Structure characteristics of freeze-dried sample of collagen oligopeptide chelated calcium was analyzed by Flourier Transform Infrared Spectrum (FT-IR), Ultraviolet Spectrum ...