期刊论文

中文

食品工业科技

1002-0306

2020

41

2

59-63

10.13386/j.issn1002-0306.2020.02.010

本校为第一机构

本研究以植物甾醇(PS)替代胆固醇与卵磷脂(PC)为原料,以粒径、多分散系数PdI、电位和包封率为指标,采用乙醇注入法制备蛋白修饰的植物甾醇脂质体(SPI-LS),通过单因素优化脂质体制备工艺,研究了SPI-LS的复溶性及其在水中的溶解度,并考察了植物甾醇包埋前后体外消化生物活性.结果 表明,最佳制备工艺为:PS: PC为1:4,乙醇体积比为25％,蛋白含量为1％;脂质体复溶后外观与冻干前无显著差异,溶解度可达1.971 mg/mL;经3h体外胃肠消化后,SPI-LS中植物甾醇的生物活性仍可保持在50％以上,是未经包埋植物甾醇的三倍之多.故优化制备条件得到的SPI-LS复溶性良好,并能有效保持植物甾醇的生物活性.

In this study,phytosterol(PS)and phosphatidylcholine(PC)were used as materials to prepare protein-modified phytosterol liposomes(SPI-LS)by ethanol injection method.Particle size,PdI,Zata potential,and the embedding rate were used to evaluate the technology conditions of preparation liposome by single factor optimization.The solubility of SPI-LS in water was observed.The bioactivity of digestion in vitro before and after PS embedding were investigated.The results showed that the optimum and technological condition were as follows:The ratio of PS...