期刊论文、会议论文

Preparation Technology Optimization and Antioxigenic Property Analysis of Phospholipids from Grass Carp Head

中文

食品工业科技

1002-0306

2020

41

6

149-154

中国食品科学技术学会第十六届年会暨第十届中美食品业高层论坛

中国食品科学技术学会第十六届年会暨第十届中美食品业高层论坛论文摘要集

2019-11-13

中国湖北武汉

10.13386/j.issn1002-0306.2020.06.025

“十三五”国家重点研发计划“蓝色粮仓科技创新”专项（2018YFD0901103）；

本校为第一机构

采用乙醇溶液浸提冷冻干燥草鱼头粉末制备磷脂,通过单因素正交试验确定最优工艺方案为:乙醇浓度80%,料液比1:6,提取温度65℃,提取时间3 h,在此条件下,得到草鱼头磷脂的纯度为83.24%,提取率为3.03%。利用薄层层析（TLC）分析比较草鱼头磷脂种类的组成及含量,利用气相色谱-质谱联用技术分析磷脂的脂肪酸组成,并进行体外抗氧化能力测定。结果表明:草鱼头磷脂主要含有磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、鞘磷脂（SM）、溶血磷脂酰胆碱（LPC）四种磷脂,其中PC的含量最高（60.00%）,其次为PE（20.00%）。草鱼头磷脂脂肪酸组成以多不饱和脂肪酸（PUFA）为主（52.09%）,其中,C20:5n-3（EPA）和C22:6...

In order to obtain higher purity grass carp head phospholipids and analyze the antioxidant properties of the prepared grass carp head phospholipids,phospholipids were prepared from freeze-dried grass head powder by ethanol extraction. After determined its optimal process by the orthogonal test,the composition and content of phospholipids from grass carp head were analyzed by thin layer chromatography( TLC). The fatty acid composition of the phospholipids was analyzed by gas chromatography-mass spectrometry( GC-MS),and the antioxidant activity i...