期刊论文

Wen, Z.-L.

中文

上海交通大学学报(医学版)

1674-8115

2011

31

11

1583-1587

10.3969/j.issn.1674-8115.2011.11.017

“十一五”国家科技重大专项基金（2009ZX10003-017和2009ZX10004-313）； 武汉工业学院2010年研究生创新基金项目（2010cx016）~~；

本校为第一且通讯机构

目的阐明武汉地区结核分枝杆菌分离株氨基糖苷类药物耐药相关基因rpsL和rrs的分子突变特征。方法从武汉医疗救治中心共收集69株结核分枝杆菌的临床菌株,包括耐链霉素(SM)菌株35株(其中4株同时对卡那霉素耐药)和SM敏感菌株34株(其中全敏感20株)。采用PCR直接测序法分析rpsL和rrs基因的分子突变特征。结果 35株SM耐药株中有26株(74.3%)检测到rpsL突变,突变位点集中在第43位和第88位密码子,并发现了1个新的突变类型Lys88Glu;13株耐药株(37.1%)检测到四种不同类型的rrs基因突变,分别为A1401G(17.1%,6/35)、A514C(14.3%,5/35)、G1487A(2.9%,1/35)和C517T(2.9%,1/35),1株全敏感菌株的rrs基因发生了C10...

Objective To characterize the rpsL and rrs genes of aminoglycoside-resistant clinical isolates of Mycobacterium tuberculosis in Wuhan. Methods Sixty-nine clinical isolates of Mycobacterium tuberculosis were collected from Wuhan Medical Treatment Center, including 35 streptomycin (SM)-resistance isolates (4 were also resistant to kanamycin) and 34 isolates susceptible to SM (20 were pan-susceptible). Mutation characteristics of rpsL and rrs genes were analyzed with PCR direct sequencing. Results Of the 35 SM-resistant clinical isolates, 26 (74.3%) carried mutations in rpsL gene, with rpsL Lys43...