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Graphene-Oxide-Based FRET Platform for Sensing Xenogeneic Collagen Coassembly

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成果类型:
期刊论文
作者:
Wei, Benmei;Zhai, Zhongwei;Wang, Haibo*;Zhang, Juntao;Xu, Chengzhi;...
通讯作者:
Wang, Haibo
作者机构:
[Xu, Yuling; Xu, Chengzhi; Zhai, Zhongwei; Xie, Dong; Wei, Benmei; Wang, Haibo; He, Lang; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Wang, Haibo] W
Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.
语种:
英文
关键词:
Energy transfer;Forster resonance energy transfer;Graphene;Thermodynamic stability;Anti-interference;Collagen fibrils;Fluorescein isothiocyanate;Fluorescence signals;Grass carp skin;Optimal conditions;Porcine skin;Sensitive method;Collagen;collagen;fluorescein isothiocyanate;graphite;oxide;animal;carp;chemistry;devices;evaluation study;fluorescence;fluorescence resonance energy transfer;genetic procedures;heat;pig;procedures;skin;Animals;Biosensing Techniques;Carps;Collagen;Fluorescein-5-isothiocyanate;Fluorescence;Fluorescence Resonance Energy Transfer;Graphite;Hot Temperature;Oxides;Skin;Swine
期刊:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN:
0021-8561
年:
2018
卷:
66
期:
34
页码:
9080-9086
基金类别:
*E-mail: wanghaibo@whpu.edu.cn. ORCID Benmei Wei: 0000-0002-7672-1707 Haibo Wang: 0000-0003-1138-898X Funding This research was supported by the National Natural Science Foundation of China (Nos. 21676208, 21706201), Hubei Provincial Nature Science Foundation of China (Nos. 2017CFB507, 2018CFA030, 2016CFB299), Foundation of Hubei Educational Commission (No. D20161703), Science and Technology Project of Wuhan City, China (No. 2016020101010082), and Wuhan Morning Light Plan of Youth Science and Technology (No. 2017050304010326). Notes The authors declare no competing financial interest. This research was supported by the National Natural Science Foundation of China (Nos. 21676208, 21706201), Hubei Provincial Nature Science Foundation of China (Nos. 2017CFB507, 2018CFA030, 2016CFB299), Foundation of Hubei Educational Commission (No. D20161703), Science and Technology Project of Wuhan City, China (No. 2016020101010082), and Wuhan Morning Light Plan of Youth Science and Technology (No. 2017050304010326).
机构署名:
本校为第一且通讯机构
院系归属:
化学与环境工程学院
摘要:
Xenogeneic collagen coassembly (XCCA) offers a new view for the design and performance regulation of novel collagen-based biomaterials. But there is still a lack of accurate and sensitive method for monitoring XCCA. In this study, a simple and efficient graphene-oxide (GO)-based fluorescence resonance energy transfer (FRET) platform has been developed to sense XCCA. We first designed a fluorescein isothiocyanate (FITC)-labeled porcine skin collagen (PSC) that adsorbed on the GO surface and effectively quenched its fluorescence. Upon the additio...

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