期刊论文

Longjie Li<&wdkj&>Hongbo Wang<&wdkj&>Xianjin Xiao

英文

Nucleic Acids Research

0305-1048

2022

50

22

12674-12688

10.1093/nar/gkac1144

This work was financially supported by the National Key Research and Development Program of China [2021YFC2701402]; National Natural Science Foundation of China [81871732]; the Open Research Fund of State Key Laboratory of Bioelectronics, South-east University [Sklb2021-k06]; Open Project Fund from NHC Key Lab of Reproduction Regulation [KF2021-02]; the Open Research Fund of State Key Laboratory of Advanced Technology for Materials Synthesis and Processing (Wuhan University of Technology) [2022-KF-2]; and the Huazhong University of Science and Technology Innovation Research Institute Science and Technology Innovation Fund [2022JYCXJJ045]. Funding for open access charge: Insititute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology.

本校为其他机构

Although CRISPR-Cas12a [clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 12a] combiningpre-amplification technology has the advantage of high sensitivity in biosensing, its generality and specificity are insufficient, which greatly restrains its application range. Here, we discovered a new targeting substrate for LbaCas12a (Lachnospiraceae bacterium Cas12a), namely double-stranded DNA (dsDNA) with a sticky-end region (PAM-SE+ dsDNA). We discovered that CRISPR-Cas12a hadspecial enzymatic properties for this substrate DNA, including the ability to reco...