Simultaneous nitrification and denitrification (SND) is cost-effective, and therefore it is a promising process for biological nitrogen removal. In this study, SND microbes were obtained through domestication of the sludge from Wuhan municipal wastewater treatment plant to enrich effective nitrogen removal SND microbes, to reveal the microbial structure of SND and the denitrogenation mechanism through the high-throughput sequencing technology and quantitative PCR. When NH4+-N (similar to 90.7 mg/) as a sole nitrogen source, the removal efficiency (RE) of NH4+-N in 24 h was 99.35%, and there wa...