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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Shi, Meng;Kong, Demin;Zhang, Hui;Rao, Deming;Zhao, Tianlong;...

通讯作者：

Wu, Jing;Wang, L

作者机构：

[Zhang, Hui; Rao, Deming; Liu, Zhanzhi; Chen, Sheng; Shi, Meng; Wu, Jing; Kong, Demin; Yang, Jing; Wang, Lei; Zhao, Tianlong] Jiangnan Univ, Sch Biotechnol, 1800 Lihu Ave, Wuxi 214122, Peoples R China.

[Zhang, Hui; Rao, Deming; Liu, Zhanzhi; Chen, Sheng; Wu, Jing; Shi, Meng; Kong, Demin; Yang, Jing; Wang, Lei; Zhao, Tianlong] Jiangnan Univ, State Key Lab Food Sci & Resources, 1800 Lihu Ave, Wuxi 214122, Peoples R China.

[Zhang, Hui; Rao, Deming; Liu, Zhanzhi; Chen, Sheng; Shi, Meng; Wu, Jing; Kong, Demin; Yang, Jing; Wang, Lei; Zhao, Tianlong] Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, 1800 Lihu Ave, Wuxi 214122, Peoples R China.

[Zhang, Hui; Liu, Zhanzhi; Chen, Sheng; Shi, Meng; Wu, Jing; Kong, Demin; Yang, Jing; Wang, Lei; Zhao, Tianlong] Jiangnan Univ, Int Joint Lab Food Safety, 1800 Lihu Ave, Wuxi 214122, Peoples R China.

[Rao, Deming] Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan 430023, Peoples R China.

通讯机构：

[Wu, J; Wang, L ] J

Jiangnan Univ, State Key Lab Food Sci & Resources, 1800 Lihu Ave, Wuxi 214122, Peoples R China.

语种：

英文

关键词：

Heterologous expression;Latex clearing protein;Molecular modification

期刊：

International Journal of Biological Macromolecules

ISSN：

0141-8130

年：

2024

卷：

254

期：

Pt 3

页码：

127995

DOI：

10.1016/j.ijbiomac.2023.127995

基金类别：

National Key Research and Development Program of China [2019YFA0706900]; Jiangsu Provincial Science and Technology Department Policy Guidance Program-International Cooperation Projects-Innovation cooperation project of "BR" [BZ2020010]; Science and Technology Project of Jiangsu Province [BE2021625]; Taishan Industrial Experts Program [tscx202306145]; Yellow River Delta Industrial Experts Program [DYRC20200205]; China Postdoctoral Science Foundation [2023M731167]

机构署名：

本校为其他机构

摘要：

Latex clearing protein from Streptomyces sp. strain K30 (Lcp(K30)) is a natural oxidoreductase that can catalyse the cleavage of rubber through dioxygenation. It has significant potential applications in polymer degradation. However, its limited expression in engineered strains restricts its utility. This study aimed to enhance the soluble expression and enzyme activity of Lcp(K30) in E. coli BL21 (DE3) by optimizing fermentation conditions and making molecular modifications. The enzyme activity reached 5.05U·mL(-1) by optimizing the induction...

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