Objective: In this paper, papain with the chitosanase activity was separated by ultra-filtration, the technical conditions of ultra-filtration were studied and the effects of pressure and concentration times on ultrafiltration permeate flux, activity and the yield rate were investigated, Conclusion:The results showed when papain was separated in the room temperature, 0.5 % of concentration, 0.1 MPa of pressure and eight times concentrated liquid, the specific activity of protease and chitosanase on papain increased to 531.39 U/mg and 2.65 U/m...