期刊论文

中文

食品与机械

1003-5788

2013

29

5

164-168

湖北省教育厅高校产学研合资助项目（编号:C2010040）； 武汉轻工大学研究生教育创新基金重点资助项目（编号:2012cx001）；

本校为第一机构

为了能够对鱼骨中蛋白质进行有效的提取,制备纯度相对较高的胶原蛋白肽,研究鱼骨中钙的脱除工艺并进行优化。试验以白鲢鱼骨为原料,采用钙脱除率和蛋白回收率为指标,研究温度、盐酸浓度、脱除时间、料液比对白鲢鱼骨中钙脱除效果的影响。根据单因素试验结果,对鱼骨中钙脱除工艺进行三因子二次正交旋转组合设计的优化,得到鱼骨中钙脱除工艺的最佳参数为温度43.3℃、盐酸浓度5.9%、脱除时间30 min、料液比1:5.5(m:m),该条件下钙的脱除率为81.0%,蛋白质回收率为70.5%。

In order to extract protein effectively from the fishbone and prepare relatively pure collagen peptides, the removal technology and its parameters of calcium in fish bones were investigated and optimized. Used silver carp fish bone as raw material, and used calcium removal rate and protein recovery rate as indexes, the effects were assayed of temperature, concentration of hydrochloric acid, time, and ratio of solid to liquid on removal of silver carp fish bone calcium, According to the single factor experiment results, the process of silver car...