期刊论文

Determination of total polyphenolsin barley spent gains by Folin-Ciocalteu colorimetric method

中文

食品与机械

1003-5788

2016

32

2

51-54

10.13652/j.issn.1003-5788.2016.02.013

本校为第一机构

采用Folin-Ciocalteu比色法测定大麦糟中总酚含量,以没食子酸为参照品确定最佳测定条件。结果表明,Folin-Ciocalteu用量1.0 mL,8% Na_2CO_3用量3.0 mL,显色温度25 ℃,反应时间60 min,避光,检测波长760 nm,没食子酸浓度在1~8 μg/mL时与吸光度具有良好线性关系,其回归方程为y=0.121x+0.017 1,R~2=0.999 2。该方法操作简单、稳定性好、准确度高,可用于大麦多酚的定量分析。

The total polyphenol content in barley spent gains were analyzed by Folin-Ciocalteu method. The optimum conditions were investigated using gallic acid as standard. and were as followed: Folin-Ciocalteu reagent 1.0 mL, 8% Na_2CO_3 3.0 mL, developing temperature 25 ℃, reaction time 60 min, in darkness. Then the solution was detected at the wavelength of 760 nm. When the gallic acid was in range of 0~8 μg/mL, the concentration and the absorbance value had a good linear relationship. The regression equation was found as followed: y=0.121x+0.017 1...