期刊论文

中文

现代食品科技

1673-9078

2020

36

8

15-22

10.13982/j.mfst.1673-9078.2020.8.1278

“十三五”重点研发计划项目（2017YFC1600100）；

本校为第一机构

本研究将初始菌株副溶血弧菌F7经过环丙沙星连续体外诱导得到的实验室诱导株H128、H256、H512作为研究对象,研究了菌株F7、H128、H256、H512的药敏性、泳动能力、生长曲线、生物膜形成能力等生物学特性,分析了多药外排泵基因vme B、vme D、vpo C、vmr A的相对表达量。结果表明,诱导菌株不仅对诱导抗生素环丙沙星的耐药性明显提高,还对多种非诱导抗生素呈现交叉耐药。与初始菌株F7相比,诱导菌株H128、H256、H512泳动能力下降,泳动圈直径分别为4.83 mm、4.57 mm、5.13 mm,生物膜形成能力增强,吸光值分别为4.35 nm、4.35 nm、4.29 nm。诱导菌株H128、H256、H512均检测到外排泵基因vme D的相对表达量...

In this study, the laboratory-induced strains H128, H256 and H512 obtained from the initial strains of Vibrio parahaemolyticus F7 after continuous in vitro induction with ciprofloxacin were used as the research objects. The biological characteristics of F7, H128, H256, H512 strains, including drug sensitivity, mobility, growth curve and biofilm-forming ability were examined. The relative expressions levels of multidrug efflux efflux pump genes, vmeB, vmeD, vpoC and vmrA were analyzed. The results showed that all the laboratory-induced strains h...