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The Alcoholic Extract of Euryale ferox Seed Shell Inhibits the Proliferation of Human Gastric Cancer SGC7901 Cells and Human Hepatoma HepG2 Cells and Promotes Their Apoptosis [芡实壳醇提物抑制人胃癌 SGC7901 细胞和人肝癌HepG2 细胞增殖并促进其凋亡]

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成果类型:
期刊论文
作者:
殷东杰;薛梦恒;杨过;黄永康;易阳;...
作者机构:
[殷东杰; 薛梦恒; 杨过; 黄永康] School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China
School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
Hubei Engineering Research Center for Fresh Food, Wuhan, 430023, China
[易阳] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Hubei Engineering Research Center for Fresh Food, Wuhan, 430023, China
[王宏勋; 王丽梅] School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Hubei Engineering Research Center for Fresh Food, Wuhan, 430023, China
语种:
中文
关键词:
芡实壳;人胃癌SGC7901细胞;人肝癌HepG2细胞;细胞增殖;细胞凋亡
关键词(英文):
Euryale ferox seed shell;human gastric cancer SGC7901 cells;human hepatoma HepG2 cells;cell proliferation;apoptosis
期刊:
现代食品科技
ISSN:
1673-9078
年:
2022
卷:
38
期:
6
页码:
55-65
基金类别:
2020BBB074:湖北省重点研发计划项目 2013d11:武汉轻工大学校立重点项目
机构署名:
本校为第一机构
院系归属:
食品科学与工程学院
生命科学与技术学院
摘要:
为明确芡实壳醇提物对SGC7901及HepG2细胞体外抑制作用及机制。该研究采用75%乙醇对芡实壳超声提取,采用福林酚法测定醇提物总酚含量,采用CCK-8法检测醇提物对SGC7901和HepG2细胞的增殖作用抑制率并计算IC50值,使用流式细胞仪检测细胞凋亡、细胞周期、细胞线粒体膜电位及胞内钙离子浓度。结果表明,醇提物对SGC7901和HePG2细胞具有增殖抑制作用,浓度为200μg/mL的醇提物作用细胞48 h后,抑制率分别为92.63%和72.40%。细胞周期检测表明,浓度为200~800μg/mL的醇提物可使SGC7901细胞阻滞于G0/G1期;浓度为50~200μg/mL的醇提物可使HepG2细胞阻滞于S期。细胞线粒体膜电位测定表明,醇提物可...
摘要(英文):
To clarify the in vitro inhibitory effect and mechanism of the alcoholic extract from Euryale ferox seed shell on human stomach cancer SGC7901 and human liver cancer HepG2 cells. In this study, 75% ethanol was used for ultrasonic extraction of Euryale ferox seed shell and the Folin-Phenol method was used to determine the content of total phenolics; the cell counting Kit-8 (CCK-8) was used to detect the inhibitory rates of the alcoholic extract on the proliferation of SGC7901 and HepG2 cells, and then the IC50 values were calculated. The flow cy...

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