期刊论文

Zou, Linling

英文

Talanta

0039-9140

2016

146

749-753

10.1016/j.talanta.2015.06.030

Jianghan University [08010001]; Hubei Province Innovative Young Research Team in Universities [T201318, T201420]; Natural Science Foundation of Hubei ProvinceNatural Science Foundation of Hubei Province [2014CFA098]; National High Technology Research and Development Program of China (863 Program)National High Technology Research and Development Program of China [2015AA033406]; Science and Technology Project of Wuhan City [2013061001010481]; Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [U1261204]

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This paper describes a promising micro-channel multiplex immunoassay method based on the quantum dots encoded beads which requires micro-volume sample. Briefly, Au nanoparticles coated polystyrene (PS) beads were prepared and Quantum dots (QDs) were employed to encode 4 types of the PS beads by different emission wavelength QDs and various intensities. Different coding types of the beads were immobilized with different antibodies on the surface and BSA was used to block the unsatisfied sites. The antibody linked beads were then arranged in the 150 μm diameter optical capillary where the spe...