期刊论文

Effect and mechanism of rosuvastatin preconditioning on inflammatory cytokines release in vascular smooth muscle cells of middle cerebral artery of rats after focal cerebral ischemia-reperfusion

Wang, X.

中文

解剖学报

0529-1356

2019

50

6

713-717

10.16098/j.issn.0529-1356.2019.06.002

(Q20121813):湖北省教育厅科学技术研究计划优秀中青年人才项目

本校为第一机构

目的 探讨瑞舒伐他汀预处理对大鼠局灶性脑缺血再灌注后大脑中动脉血管平滑肌细胞(VSMCs)中炎性细胞因子白细胞介素(IL)-1β、IL-6、肿瘤坏死因子α(TNF-α)表达的影响以及可能的机制.方法 36只健康成年SD大鼠,雌雄不限,随机分为假手术组,局灶性脑缺血再灌注组,瑞舒伐他汀预处理组,每组12只.大脑中动脉缺血2h再灌注24 h后,Real-time PCR及Western blotting法检测大鼠大脑中动脉VSMCs中IL-1β、IL-6和TNF-α以及核因子κB(NF-κB) mRNA和蛋白的表达.结果 再灌注24 h后,模型组VSMCs IL-1β、IL-6以及TNF-αmRNA和蛋白的表达明显增加,而给予瑞舒伐他汀预处理后,可明显抑制大脑中动脉VSMCs中IL-1β、...

Objective To explore the effect and probable mechanism of rosuvastatin preconditioning on inflammatory cytokines interleukin (IL) -1β, IL-6 and tumor necrosis factor a (TNF-α) release in vascular smooth muscle cells (VSMCs) of middle cerebral artery after ischemia-reperfusion. Methods Thirty-six healthy SD rats were randomly assigned into three groups; sham operation group, focal cerebral ischemia-reperfusion group and rosuvastatin preconditioning group. There were 12 rats in each group. At the 24th hour of reperfusion after middle cerebral a...