期刊论文

Interaction of Propolis Flavonoids and CTDNA

中文

食品研究与开发

1005-6521

2015

11

26-28

10.3969/j.issn.1005-6521.2015.11.006

本校为第一机构

研究蜂胶黄酮与ctDNA的相互作用及方式;采用紫外-可见吸收光谱法,通过比较不同浓度ctDNA与蜂胶黄酮化合物反应后的吸光度变化,根据吸收曲线得出黄酮化合物与ctDNA在不同条件下的结合常数;加入ctDNA后蜂胶黄酮的吸光强度明显下降,出现减色效应,不同pH条件下,黄酮和ctDNA的结合常数也不同;蜂胶黄酮可以和ctDNA发生有效结合,结合强度受pH变化的影响。

Explore the interaction and mode between propolis flavonoids and ctDNA. Through the UV-visible absorption spectroscopy measure, comparing the absorbance intensity changes of flavonoids after react with different concentrations ctDNA to analysis the binding constant between propolis and ctDNA. The absorption intensity of propolis decreased when add ctDNA in the sample. The binding constants of flavonoids and ctDNA were also different in different pH conditions. There was an interaction between propolis fla...