期刊论文

Determination of physicolchemical properties of sterically stabilized immunoliposomal islet neogenesis-associated protein

中文

哈尔滨医科大学学报

Journal of Harbin Medical University

1000-1905

2009

43

6

527-531

10.3969/j.issn.1000-1905.2009.06.001

国家自然科学基金资助项目(30671000)；

本校为其他机构

目的 以肝细胞生长因子为包被对象,研究胰岛新生相关蛋白(INGAP)空间稳定免疫脂质体的制备方法 、理化性质和体外靶向性.方法 制备含PDP-PEG-HSPE的空间稳定脂质体,共价连接马来酰亚胺衍生化IN-GAP.激光散射粒度仪测定其粒径分布,比色法测定包封率,透析法检测药物释放度及累积泄漏率,ELISA法检测脂质体表面的抗体免疫活性,流式细胞仪及免疫荧光考察该免疫脂质体对ARIP细胞(大鼠胰腺导管癌细胞系)的结合活性.结果 INGAP-SIL[HGF]脂质体的粒径分布为(95.86±6.54)nm;包封率为(88.37±3.58)%;24 h释放度为(23.26±2.45)%;4 ℃保存时7 d内药物累积泄漏率小于3%;4℃和25℃条件下保存7 d,免疫活性均在...

Objective To study the preparation methods,physical and chemical properties of the islet neogenesis-associated protein （INGAP） sterically stabilized immunoliposomes. Methods An endgroup functionalized polyethylene glycol-lipid deirvative （pyridylthiopropionoylamino-polyethylene glycol-hydrogenated soy phosphatidylethanolamine,PDP-PEG-HSPE） was synthesized and incorporated to sterically stabilized liposomes. After mild thiolysis of the PDP groups by dithiothreitol,liposomes were covalently linked with maleimide-derivatized INGAP and formed s...