A Novel Fluorescence cross-priming Amplification Based on Universal Molecular Beacon for Rapid and Specific Detection of Salmonella enterica in food Samples

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Hou, Wenfu;Du, Juan;Liu, Tingting;Wang, Wensi;Ai, Youwei;...

通讯作者：

Hou, WF;Wang, Z

作者机构：

[Hou, WF; Liu, Tingting; Zhou, Min; Hou, Wenfu; Du, Juan; Wang, Wensi; Ai, Youwei] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.

[Wang, Hongxun] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

[Hou, WF; Hou, Wenfu; Ai, Youwei] Inst Hubei Jinchu Food Ind Technol Res, Jinzhou, Peoples R China.

[Wang, Zhuo] Fifth Peoples Hosp Shanaxi Prov, Xian 610110, Shanaxi, Peoples R China.

[Liu, Tingting; Zhou, Min; Du, Juan; Wang, Wensi] Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

通讯机构：

[Hou, WF ] W

[Wang, Z ] F

Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.

Inst Hubei Jinchu Food Ind Technol Res, Jinzhou, Peoples R China.

Fifth Peoples Hosp Shanaxi Prov, Xian 610110, Shanaxi, Peoples R China.

语种：

英文

关键词：

Molecular Beacon;Cross-priming Amplification;Salmonella;Rapid Detection

期刊：

Food Analytical Methods

ISSN：

1936-9751

年：

2024

卷：

期：

页码：

1394-1401

DOI：

10.1007/s12161-024-02638-1

基金类别：

The Knowledge Innovation Special Fundamental Research Project of Wuhan [2022020801010390]; Knowledge Innovation Special Fundamental Research Project of Wuhan [2021BED 006]; Science and Technology Project of Hubei Province

机构署名：

本校为第一且通讯机构

院系归属：

食品科学与工程学院

生命科学与技术学院

摘要：

A methodology with rapidity and specificity is of great significance for the effective control and management of outbreaks caused by Salmonella enterica as it has presented an obvious threat to food safety and public health worldwide. In this study, we aimed to develop a tube-closed and real time cross-priming amplification assay for the rapid and sensitive detection of S.enterica and decrease the possibility of detecting false-positive signals derived from aerosol contamination or non-specific amplification caused by traditional CPA assay. The reaction system was optimized and the results sho...

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A Novel Fluorescence cross-priming Amplification Based on Universal Molecular Beacon for Rapid and Specific Detection of Salmonella enterica in food Samples

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