期刊论文

中文

中国油脂

1003-7969

2007

32

8

38-40

10.3321/j.issn:1003-7969.2007.08.009

本校为第一机构

采用pH-stat法测定水解度,探讨了两种蛋白酶分步水解棉籽蛋白的反应条件和影响因素.实验结果表明,先使用碱性蛋白酶Alcalase,在酶加量为750 U/g、水解温度60 ℃、底物浓度5%、pH为8的条件下水解300min;灭酶后再加入中性蛋白酶AS1.398,在酶加量8 000 U/g、水解温度60 ℃、pH为7的条件下再水解100 min;两种酶的最终水解度达到20.08%.

Cottonseed protein was stepwise hydrolyzed by two kinds of enzyme and the degree of hydrolysis （DH） was determined by pH-stat method. The reaction conditions and influence factors were researched. The results showed that cottonseed protein was first hydrolyzed by Alcalase under conditions as follows： enzyme dosage 750 U/g,temperature 60 ℃,substrate concentration 5%, pH 8,time 300 minutes. The hydrolysate was treated to exterminate the power of Alcalase,and then hydrolyzed by neutral protease AS1.398 under conditions as follows： enzyme dosa...