期刊论文

中文

中国油脂

1003-7969

2010

35

10

21-24

湖北省2008年度重大科技专项（ZDN0005）；

本校为第一机构

采用碱提酸沉法从低变性脱脂花生粕中提取花生分离蛋白.探讨了碱提温度,碱提时间,碱提固液比等因素对产品蛋白质含量和得率的影响.通过正交试验优化制备花生分离蛋白的最佳工艺条件为:碱提温度55℃,碱提2次,每次1.5 h,碱提固液比1:9,碱提pH 9.0,酸沉pH 4.5.在此条件下产品的蛋白质含量为90.87%(N×6.25,干基),得率最高可达38.65%.该产品的氮溶解指数为68.46%,持水性2.3 g/g,吸油性1.3 mL/g,乳化性50.4%,乳化稳定性56.55%

The alkali-extraction and acid-precipitation process was used to prepare peanut protein isolates from low-denatured defatted peanut meal.The effects of alkali-extraction temperature,time and solid-liquid ratio on the protein content and yield of the product were investigated.Through orthogonal experiments,the optimum conditions were obtained as follows：alkali-extraction temperature 55 ℃,times 2 and once for 1.5 h,solid-liquid ratio 1∶9,alkali-extraction pH 9.0,acid-precipitation pH 4.5.Under these conditions,the product yield was 38.65%,and ...