Peony seed protein was prepared by alkali extraction and acid preclpltanon process w,m ~,u- coamylase assisted. The results showed that the high purity peony seed protein was obtained with glu- coamylase assisted, which could achieve the requirement of protein isolate. The optimal process condi- tions of preparation of peony seed protein were as follows: solid liquid ratio 1: 10, alkali extraction pH 9.5, acid precipitation pH 4.5, glucoamylase reaction time 30 min, glucoamylase dosage 0. 03% , glucoamylase reaction pH 4. 0. Under the optimal...