期刊论文

中文

中国油脂

1003-7969

2014

39

12

72-76

本校为第一机构

为了研究高山被孢霉突变株12-2-2产花生四烯酸(ARA)的培养条件,选取温度、pH、摇床转速和培养时间为4个影响因素,以生物量、油脂产量和ARA产量为综合评价指标,进行单因素实验和正交实验。结果表明,影响高山被孢霉突变株12-2-2产ARA的因素主次顺序为:温度﹥培养时间﹥摇床转速﹥pH;最优培养条件为:温度26℃,培养时间7 d,摇床转速160 r/min,pH 8。在最优培养条件下,高山被孢霉突变株12-2-2的生物量为32.31 g/L,油脂产量为12.98 g/L,ARA产量为5.12 g/L。

With temperature, pH, shaking speed and culture time as four influencing factors, and bio- mass and productivities of oil and arachidonic acid （ARA） as comprehensive evaluation indexes, the cul- ture conditions of Mortierella alpina mutant 12 - 2 - 2 to produce ARA were studied by single factor ex- periment and orthogonal experiment. The results showed that temperature had the greatest influence on the ARA production by MortiereUa alpina mutant 12 -2 -2, followed by culture time, shaking speed and pH; the optimal culture conditions were obtai...