期刊论文

Simultaneous extraction of Xanthoceras sorbifolia Bunge oil and protein by aqueous enzymatic method

中文

中国油脂

1003-7969

2016

41

9

1-6

10.3969/j.issn.1003-7969.2016.09.001

国家粮食局粮食公益性行业科研专项（201313012-03）；

本校为第一机构

采用水酶法同时提取文冠果油及蛋白质,以油脂得率及蛋白质得率为指标,采用单因素实验对酶种类、酶解pH、酶解温度、料液比、酶解时间与加酶量6个因素进行研究,并通过正交实验得到最佳工艺。实验得到的最佳酶解条件为:果胶酶与纤维素酶复合酶配比2:1,酶解pH 5,酶解温度50℃,料液比1:5,酶解时间4 h,加酶量1 500 U/g。在最佳条件下油脂得率为35.41% ,蛋白质得率为14.43%。文冠果油脂肪酸测定结果表明,文冠果油中含棕榈酸4. 60%,硬脂酸2. 12%,油酸31.28%,亚油酸40.86%,芥酸8.88%。文冠果蛋白氨基酸测定结果表明,文冠果蛋白氨基酸种类齐全,其中谷氨酸含量最高(占氨基酸总量的18. 86%),其次为丝氨酸和...

Xanthoceras sorbifolia Bunge oil and protein were simultaneously extracted by aqueous enzymatic method. With yields of oil and protein as indexes, six factors （ types of enzyme, enzymolysis pH, enzymolysis temperature, ratio of solid to liquid, enzymolysis time and enzyme dosage） were studied by single factor experiment, and the extraction conditions were optimized by orthogonal experiment as follows：ratio of pectinase to cellulose 2 ： 1, enzymolysis pH 5, enzymolysis temperature 50 ~C, ratio of solid to liquid 1 ： 5, enzymolysis time 4 h ...