期刊论文

Preparation of coconut fractionated protein

中文

中国油脂

1003-7969

2019

44

5

67-72

10.3969/j.issn.1003-7969.2019.05.016

本校为第一机构

通过改良的Osboren法对脱脂椰子粉进行分级提取,得到清蛋白、球蛋白、醇溶蛋白和谷蛋白,并对4种椰子蛋白进行氨基酸组成分析和SDS-PAGE电泳分析。在单因素实验基础上,利用正交实验对4种椰子蛋白提取条件进行优化。结果表明,4种椰子蛋白最佳提取工艺条件为:在液料比10∶1、提取时间4 h、提取温度50℃条件下,清蛋白提取率为49. 05%,纯度为57. 1%;在液料比10∶1、提取时间4 h、提取温度50℃条件下,球蛋白提取率为46. 65%,纯度为70. 8%;在液料比10∶1、提取时间5 h、提取温度55℃、乙醇体积分数75%条件下,醇溶蛋白提取率为18. 01%,纯度为32. 7%;在液料比10∶1、提取时间4 h、提取温度50℃、碱溶p H ...

The defatted coconut powder was fractionated and extracted by modified Osboren method to obtain albumin,globulin,alcohol soluble protein and gluten,and their amino acid compositions and SDS - PAGE electrophoresis were analyzed. Based on single factor experiment,the preparation conditions of the four kinds of coconut proteins were optimized by orthogonal experiment. The results showed that the optimal preparation conditions of the four kinds of coconut proteins were obtained as follows: ①ratio of liquid to material 10∶ 1,extraction time 4 h an...