期刊论文

Characterization and application of carbon dots from rice bran protein

中文

中国油脂

1003-7969

2022

47

2

58-64

10.19902/j.cnki.zgyz.1003-7969.210157

生物医学分析化学教育部重点实验室（武汉大学）开放课题（No. ACBM2019003）； 湖北省科技重大专项，2019ABA105； 湖北省粮食科技创新项目；

本校为第一机构

以米糠蛋白(RBP)为碳源,采用水热合成法制备碳量子点,通过紫外-可见吸收光谱、荧光光谱、高分辨率透射电子显微镜(TEM)、傅里叶变换红外光谱(FTIR)对制备的米糠蛋白碳量子点(RBP-CDs)的光学性能、形貌结构及表面官能团进行表征,并探究RBP-CDs在不同环境下的稳定性及应用于传感、细胞成像、荧光染色等领域的效果。结果表明:RBP-CDs在286 nm下有紫外吸收峰,其发射波长具有激发依赖性;RBP-CDs的形状近似球形,分散性好,尺寸均一,粒径为2~4 nm;RBP-CDs表面富含羟基、羧基,亲水性好;在pH 4~10及不同盐浓度条件下,RBP-CDs的荧光性能良好;在365 nm紫外灯长时间照射下,也表现出良好的光学稳定性,但在不同...

The carbon dots (CDs) were prepared by hydrothermal method using rice bran protein(RBP) as the carbon source. The optical properties, morphology and surface functional groups of the prepared rice bran protein carbon dots (RBP-CDs) were characterized by UV-vis absorption spectrum, fluorescence spectrum, high-resolution transmission electron microscope (TEM) and Fourier transform infrared spectroscopy (FTIR), the stability of RBP-CDs in different environments and their application in sensing, cell imaging and fluorescent staining were investigate...