期刊论文

中文

中国油脂

1003-7969

2023

48

12

121-127

10.19902/j.cnki.zgyz.1003-7969.220538

2021CFB224:湖北省自然科学基金 BK20200734:江苏省自然科学基金 2021Y06:武汉轻工大学科研项目 T2020012:湖北省高校优秀中青年科技创新团队 2020BBA043:湖北省重点研发项目

本校为第一机构

为了促进黄丝藻胞内储能物质的高效积累,研究了不同氮形态(NO3-、Urea和NH4+)及初始氮浓度(1、3、6、9、18 mmol/L)对黄丝藻生长、碳水化合物和总脂积累的影响,在此基础上建立了基于氮调控促进黄丝藻生物量和储能物质同步高效积累的"低氮(初始氮浓度3 mmol/L)转高氮(初始氮浓度18 mmol/L)"两步法培养策略,并对两步法培养策略的培养时间进行优化.结果表明:与Urea和NH4+比较,黄丝藻更倾向于利用NO3-,分别在3 mmol/L和18 mmol/L条件下获得最高生物量(3.69 g/L)和总脂含量(57.3％);在"低氮转高氮"两步法培养中,低氮培养时间6 d转高氮培养12 d可显著提高黄丝藻胞内储能物质的积累,储能物质产率最高,达...

In order to promote efficient enrichment of energy storage compounds in Tribonema sp.,the effects of different nitrogen forms(NO3-,Urea and NH4+)and initial nitrogen concentration(1,3,6,9,18 mmol/L)on the growth,carbohydrate and lipid accumulation of Tribonema sp.were investigated.Then a two-step culture strategy from low nitrogen(initial nitrogen concentration 3 mmol/L)to high nitrogen(initial nitrogen concentration 18 mmol/L)based on nitrogen regulation was established to promote the simultaneous and efficient accumulation of biomass and ener...